Volkov, Solomon. <i>El coro mágico. Una historia de la cultura rusa de Tólstoi a Solzhenitsyn</i> : Barcelona, Ariel, 2010. pags. 384

El Coro Mágico pretende ser una obra única en su tipo. Desde el comienzo deja en claro que su propósito no es explicar de modo enciclopédico ciertos temas que asume el lector conoce ni presentar la vida de personajes de modo acabado, sino que más bien persigue una articulación de toda esta información sobre rieles temáticos y temporales.Facultad de Periodismo y Comunicación Socia

Volkov, Solomon. <i>El coro mágico. Una historia de la cultura rusa de Tólstoi a Solzhenitsyn</i> : Barcelona, Ariel, 2010. pags. 384

El Coro Mágico pretende ser una obra única en su tipo. Desde el comienzo deja en claro que su propósito no es explicar de modo enciclopédico ciertos temas que asume el lector conoce ni presentar la vida de personajes de modo acabado, sino que más bien persigue una articulación de toda esta información sobre rieles temáticos y temporales.Facultad de Periodismo y Comunicación Socia

Volkov, Solomon. <i>El coro mágico. Una historia de la cultura rusa de Tólstoi a Solzhenitsyn</i> : Barcelona, Ariel, 2010. pags. 384

El Coro Mágico pretende ser una obra única en su tipo. Desde el comienzo deja en claro que su propósito no es explicar de modo enciclopédico ciertos temas que asume el lector conoce ni presentar la vida de personajes de modo acabado, sino que más bien persigue una articulación de toda esta información sobre rieles temáticos y temporales.Facultad de Periodismo y Comunicación Socia

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

The present study describes the behavior of a natural phenanthrene-degrading consortium (CON), a synthetic consortium (constructed with isolated strains from CON) and an isolated strain form CON (Sphingobium sp. AM) in phenanthrene cultures to understand the interactions among the microorganisms present in the natural consortium during phenanthrene degradation as a sole carbon and energy source in liquid cultures. In the contaminant degradation assay, the defined consortium not only achieved a major phenanthrene degradation percentage (> 95%) but also showed a more efficient elimination of the intermediate metabolite. The opposite behavior occurred in the CON culture where the lowest phenanthrene degradation and the highest HNA accumulation were observed, which suggests the presence of positive and also negative interaction in CON. To consider the uncultured bacteria present in CON, a metagenomic library was constructed with total CON DNA. One of the resulting scaffolds (S1P3) was affiliated with the Betaproteobacteria class and resulted in a significant similarity with a genome fragment from Burkholderia sp. HB1 chromosome 1. A complete gene cluster, which is related to one of the lower pathways (meta-cleavage of catechol) involved in PAH degradation (ORF 31–43), mobile genetic elements and associated proteins, was found. These results suggest the presence of at least one other microorganism in CON besides Sphingobium sp. AM, which is capable of degrading PAH through the meta-cleavage pathway. Burkholderiales order was further found, along with Sphingomonadales order, by a metaproteomic approach, which indicated that both orders were metabolically active in CON. Our results show the presence of negative interactions between bacterial populations found in a natural consortium selected by enrichment techniques; moreover, the synthetic syntrophic processing chain with only one microorganism with the capability of degrading phenanthrene was more efficient in contaminant and intermediate metabolite degradation than a generalist strain (Sphingobium sp. AM).Centro de Investigación y Desarrollo en Fermentaciones Industriale

Assigning ecological roles to the populations belonging to a phenanthrene-degrading bacterial consortium using omic approaches

The present study describes the behavior of a natural phenanthrene-degrading consortium (CON), a synthetic consortium (constructed with isolated strains from CON) and an isolated strain form CON (Sphingobium sp. AM) in phenanthrene cultures to understand the interactions among the microorganisms present in the natural consortium during phenanthrene degradation as a sole carbon and energy source in liquid cultures. In the contaminant degradation assay, the defined consortium not only achieved a major phenanthrene degradation percentage (> 95%) but also showed a more efficient elimination of the intermediate metabolite. The opposite behavior occurred in the CON culture where the lowest phenanthrene degradation and the highest HNA accumulation were observed, which suggests the presence of positive and also negative interaction in CON. To consider the uncultured bacteria present in CON, a metagenomic library was constructed with total CON DNA. One of the resulting scaffolds (S1P3) was affiliated with the Betaproteobacteria class and resulted in a significant similarity with a genome fragment from Burkholderia sp. HB1 chromosome 1. A complete gene cluster, which is related to one of the lower pathways (meta-cleavage of catechol) involved in PAH degradation (ORF 31–43), mobile genetic elements and associated proteins, was found. These results suggest the presence of at least one other microorganism in CON besides Sphingobium sp. AM, which is capable of degrading PAH through the meta-cleavage pathway. Burkholderiales order was further found, along with Sphingomonadales order, by a metaproteomic approach, which indicated that both orders were metabolically active in CON. Our results show the presence of negative interactions between bacterial populations found in a natural consortium selected by enrichment techniques; moreover, the synthetic syntrophic processing chain with only one microorganism with the capability of degrading phenanthrene was more efficient in contaminant and intermediate metabolite degradation than a generalist strain (Sphingobium sp. AM).Centro de Investigación y Desarrollo en Fermentaciones Industriale

Archaeoproteomics as a complement of paleopathological studies in human skeletal remains of Cueva de Plaza, Chubut, Argentina: scope and limitations

En este trabajo se propone realizar por primera vez en Argentina un análisis proteómico por espectrometría de masas de una falange humana con una alteración macroscópica proveniente de un conjunto arqueológico recuperado en el sitio Cueva de Plaza (Chubut, Argentina). Se presentan dos métodos de extracción de proteínas de restos óseos arqueológicos para ser analizadas con un espectrómetro de masas. Ambos métodos permitieron obtener resultados no excluyentes con información parcialmente redundante y complementaria. Se identificaron proteínas como colágenos, fibronectina, proteínas de cartílago, de hueso, de músculo y de sangre y conjuntos de proteínas asociadas al sistema inmune y a otras vías metabólicas, en menor cantidad. Las proteínas identificadas son consistentes con la existencia de un trauma en proceso de reparación en el elemento óseo analizado.In this work a proteomic analysis by mass spectrometry of a human phalanx with a macroscopic alteration found in Cueva de Plaza archaeological site (Chubut, Argentina) is proposed, representing the first time that such analysis has been carried out on archaeological materials in Argentina. Two methods of protein extraction from archaeological bone remains are presented and their results were analyzed with a mass spectrometer. Both methods yielded non-exclusive, partially redundant and complementary results. Collagens, fibronectin, proteins from cartilage, bone, muscle and blood and, to a lesser extent, proteins associated with the immune system and other metabolic pathways were identified. These proteins are consistent with the existence of a healing trauma process in the analyzed bone element.Asociación de Antropología Biológica Argentin